Transfection ofBacillus subtiliswith phage Ï105 DNA: Effect of Ï105EcoRI restriction endonuclease fragments
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چکیده
منابع مشابه
Cloning DNA restriction endonuclease fragments with protruding single-stranded ends.
A new method of in vitro recombination was employed to construct plasmids containing lac promoter fragments 64 bp and 144 bp long. The 64 bp HpaII-HhaI fragment contains the binding site for the catabolite activator protein (CAP). The HpaII-HaeIII 144 bp fragment includes the binding sites for RNA polymerase, the lac repressor and CAP. The method utilizes the ability of T4 DNA polymerase to mak...
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Transcribing Bacillus cereus DNA was visualized by means of autoradiography of electrophoretically separated EcoRI restriction endonuclease DNA fragments hybridizing 32P-labeled RNA. Hybridization of RNA of dormant spores, vegetative cells, and outgrowing spores indicates the following. (i) A large fraction of the nonribosomal RNA in dormant spores is transcribed at a limited number of regions ...
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AIMS To develop a genome based DNA fingerprinting system for Aspergillus fumigatus mould. METHODS DNA was extracted from 21 isolates obtained from eight patients with an aspergilloma. This was with a freeze-dried mycelial extract fragmented in liquid nitrogen. DNA was subsequently purified by phenol-chloroform extraction followed by ultracentrifugation on a caesium chloride gradient. The DNA ...
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OMPETENT BmiZZus subtilis cells when infected with DNA isolated from bacteriophage SP82G give rise to infective phage particles (GREEN 1964). This reaction which has been observed with a number of different phages has been called transfection (FOLDES and TRAUTNER 1964). There are two modes of transfection which depend on the bacteriophage from which the DNA was isolated. The first requires only...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1981
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1981.tb06926.x